Deinococcus Sonorensis Characterization Continuation
Introduction: As we continue our characterization of Deinococcus Sonorensis in preparation to our upcoming conference, our group detailed a schedule to work upon. This past week, we prepped and performed a starch digestion test on the bacteria. We prepared agar plates of our bacteria along with positive and negative controls, starving the colonies, to test sporulation count. Media was prepared to test for urease and protease. From there, we outlined further plans for the SIM test we plan on performing. Procedure: After initial growth of colonies on starch plates, we as a group decided upon a different strategy, as the growth from previous attempts failed to yield optimal growth required for the iodine portion of the test. 1. We took one starch plate prepared for the group, and using a sharpie, marked a division of the plate into four even quadrants. 2. We labeled each section with the indicated positive, negative, and unknown bacteria as follows: ...